A temperature-sensitive mutant of Abelson murine leukemia virus confers inducibility of IgM expression to transformed lymphoid cells.
AUTOR(ES)
Takemori, T
RESUMO
Lymphoid cell lines were isolated that were inducible for the expression of surface immunoglobulin by shift from 35.5 to 39.5 degrees C after infection of mouse bone marrow cells with a mutagen-treated Abelson murine leukemia virus. Virus produced by one of the cell lines (ts49) transmitted the temperature-sensitive phenotype to new lymphoid transformants as well as to NIH/3T3 cells. In addition, the tyrosine autophosphorylating activity of the p120gag-abl protein synthesized in ts49-transformed cells was found to be temperature-sensitive. Shift experiments using ts49-transformed lymphoid cells showed that at 39.5 degrees C they synthesize increased amounts of mu and kappa chain RNA and protein, and that they can be further induced to secrete IgM when treated with lipopolysaccharide.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=553488Documentos Relacionados
- Virus production by Abelson murine leukemia virus-transformed lymphoid cells.
- Temperature-sensitive mutants of Abelson murine leukemia virus deficient in protein tyrosine kinase activity.
- Temperature-sensitive mutants of chemically transformed epithelial cells.
- Temperature-sensitive tumorigenicity of cells transformed by a mutant of Moloney sarcoma virus.
- Temperature-sensitive cellular mutant for expression of mRNA from murine retrovirus.
