A temperature-sensitive mutant of Newcastle disease virus defective in intracellular processing of fusion protein.
AUTOR(ES)
Matsumura, H
RESUMO
A temperature-sensitive mutant (ts3) of Newcastle disease virus was physiologically characterized. All major viral structural proteins were synthesized at the permissive (37 degrees C) and nonpermissive (42 degrees C) temperatures, but the fusion (F) glycoprotein was not cleaved at 42 degrees C. In immunocytochemical electron microscopy, the F protein was abundant in the rough endoplasmic reticulum but not in cytoplasmic membrane at 42 degrees C. Noninfectious hemagglutinating virus particles containing all major structural proteins except the F protein were released at 42 degrees C from infected cells. We concluded that the defect in ts3 resides in the intracellular processing of the F protein.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=249268Documentos Relacionados
- Temperature-Sensitive Simian Virus 40 Mutant Defective in a Late Function
- Neurospora crassa temperature-sensitive mutant apparently defective in protein synthesis.
- Characterization of a temperature-sensitive influenza B virus mutant defective in neuraminidase.
- Revertant analysis of a temperature-sensitive mutant of Newcastle disease virus with defective glycoproteins: implication of the fusion glycoprotein in cell killing and isolation of a neuraminidase-deficient hemagglutinating virus.
- Virion functions of RNA+ temperature-sensitive mutants of Newcastle disease virus.
