Acceptance and transfer of R-factor RP1 by members of the "herbicola" group of the genus Erwinia.

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RESUMO

The R-factor RP1 was transferred by conjugation from Pseudomonas aeruginosa PAO12r(RPI) to various strains of Erwinia herbicola and to one strain of Erwinia stewartii. The exconjugate strains had minimum inhibitory concentration values for carbenicillin, kanamycin, neomycin, and tetracycline somewhat lower than the corresponding values for the pseudomonad RP1 donor strain. The biochemical characteristics of the exconjugant strains displayed minor variation in some instances from those of the corresponding R- strains. Sensitivity of the RP1+ strains to the RP1-specific bacteriophages PRD1 and PRR1 varied from an efficiency of plating [compared with P. aeruginosa PA067(RP1)] of 0 [E. herbicola Y46(RP1)] to 133 [E. herbicola Y190(RP1)] and 148 [E. stewartii SS104R(RP1)] for PRD1, and from 0 [E. herbicola Y46(RP1)] to 0.0002 [E. herbicola Y185(RP1)] and 18.4 [E. stewartii SS104R(RP1)] for PRR1. The phage-resistant strain E. herbicola Y46(RP1), would donate, by conjugation, the R-factor to E. herbicola Y46rifr, P, aeruginosa PAT900, or Escherichia coli UB1005 only at extremely low frequencies, if at all. Transformation of E. coli JC7620 by covalently closed circular DNA from E. herbicola Y46(RP1) gave and E. coli R+ strain exhibiting the expected antibiotic resistance pattern and having the ability to donate RP1 by conjugation. It is suggested (i) that some strains of E. herbicola RP1 either do not produce RP1 pili or produce defective pili, and (ii) that sensitivity to the bacteriophages PRD1 and PRR1 is not a suitable means of diagnosing the presence RP1 in E. herbicola strains.

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