Actin Antibody: The Specific Visualization of Actin Filaments in Non-Muscle Cells
AUTOR(ES)
Lazarides, Elias
RESUMO
Actin purified from mouse fibroblasts by sodium dodecyl sulfate gel electrophoresis was used as antigen to obtain an antibody in rabbits. The elicited antibody was shown to be specific for actin as judged by immunodiffusion and complement fixation against partially purified mouse fibroblast actin and highly purified chicken muscle actin. The antibody was used in indirect immunofluorescence to demonstrate by fluorescence light microscopy the distribution and pattern of actin-containing filaments in a variety of cell types. Actin filaments were shown to span the cell length or to concentrate in “focal points” in patterns characteristic for each individual cell.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=388433Documentos Relacionados
- Antibody to Myosin: The Specific Visualization of Myosin-Containing Filaments in Nonmuscle Cells
- Nucleotide sequence of the human gamma cytoskeletal actin mRNA: anomalous evolution of vertebrate non-muscle actin genes.
- Detection and ultrastructural localization of human smooth muscle myosin-like molecules in human non-muscle cells by specific antibodies.
- Filamin, a new high-molecular-weight protein found in smooth muscle and non-muscle cells.
- The prognostic impact of tumor necrosis in non-muscle invasive bladder cancer