Activation and centromeric localization of CCAAT/enhancer-binding proteins during the mitotic clonal expansion of adipocyte differentiation

AUTOR(ES)

Tang, Qi-Qun

FONTE

Cold Spring Harbor Laboratory Press

RESUMO

Hormonal induction of 3T3-L1 preadipocytes triggers a cascade of events that initiate differentiation into adipocytes. CCAAT/enhancer-binding proteins β and δ (C/EBPβ/δ) are expressed early in the differentiation program, but are not immediately active. After a long lag, C/EBPβ/δ become competent to bind to the C/EBP regulatory element in the C/EBPα gene promoter, C/EBPα being a transcriptional activator of numerous adipocyte genes. As C/EBPβ/δ acquire binding activity, they become localized to centromeres as preadipocytes synchronously enter S phase at the onset of mitotic clonal expansion. Localization to centromeres occurs through C/EBP consensus-binding sites in centromeric satellite DNA. C/EBPα, which is antimitotic, becomes centromere-associated much later in the differentiation program as mitotic clonal expansion ceases and the cells become terminally differentiated.

Documentos Relacionados

• CCAAT/enhancer-binding protein β is required for mitotic clonal expansion during adipogenesis
• CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials.
• Avian retroviral long terminal repeats bind CCAAT/enhancer-binding protein.
• Role of C/EBP homologous protein (CHOP-10) in the programmed activation of CCAAT/enhancer-binding protein-β during adipogenesis
• Daxx Is a Transcriptional Repressor of CCAAT/Enhancer-binding Protein β*