Activation of proteinase-activated receptor 2 activation, a signal to joint innate immune responses. / Ativação do receptor ativado por protease 2, um sinal para resposta imunológica inata na articulação temporomandibular.
AUTOR(ES)
Alexandre Denadai Souza
DATA DE PUBLICAÇÃO
2009
RESUMO
We hypothesised that the early pro-inflammatory effects of proteinase-activated receptor 2 (PAR2) activation in the temporomandibular joint (TMJ) are mediated by neurogenic mechanisms. Immunofluorescence analysis revealed a high degree of neurons expressing PAR2 in retrogradely labelled trigeminal ganglion neurons. Furthermore, PAR2 immunoreactivity was observed in the lining layer of the TMJ, co-localizing with the neuronal marker PGP9.5 and substance P-containing peripheral sensory nerve fibres. The intra-articular injection of PAR2 agonists into the TMJ triggered a dose-dependent increase in plasma extravasation, neutrophil influx and induction of mechanical allodynia. The pharmacological blockade of NK1 receptors abolished PAR2-induced plasma extravasation and inhibited neutrophil influx and mechanical allodynia. We conclude that PAR2 activation is pro-inflammatory in the TMJ, through a neurogenic mechanism involving NK1 receptors. This suggests that PAR2 is an important component of innate neuro-immune response in the TMJ.
ASSUNTO(S)
carrageenan pain substância p carragenina artrite substance p arthritis inflammation inflamação articulação temporomandibular temporomandibular joint. dor
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