Activity of the transposon Tam3 in Antirrhinum and tobacco: possible role of DNA methylation.
AUTOR(ES)
Martin, C
RESUMO
The transposon Tam3 from Antirrhinum majus can transpose in a heterologous host (Nicotiana tabacum); thus the element is autonomous, probably encoding the specific information required for its own transposition. In transgenic tobacco Tam3 rapidly becomes methylated at its ends whilst adjacent flanking sequences remain hypomethylated. This methylation may account for our failure to detect Tam3 transposition in the progeny of transgenic tobacco. Treatment with the inhibitor of cytosine methylation, 5 aza-cytosine appeared to induce transposon related activity at a low level. In Antirrhinum methylation also appears to be associated with inactivation of Tam3 copies.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=400906Documentos Relacionados
- Temperature Shift Coordinately Changes the Activity and the Methylation State of Transposon Tam3 in Antirrhinum majus
- Stable Transcription Activities Dependent on an Orientation of Tam3 Transposon Insertions into Antirrhinum and Yeast Promoters Occur Only within Chromatin1[W]
- A chromosome rearrangement suggests that donor and recipient sites are associated during Tam3 transposition in Antirrhinum majus
- Large-Scale Chromosomal Restructuring Is Induced by the Transposable Element Tam3 at the Nivea Locus of Antirrhinum Majus
- Erratum: A chromosome rearrangement suggests that donor and recipient sites are associated during Tam3 transposition in Antirrhinum majus.