Adaptações transgeracionais do pâncreas endócrino em camundongos provenientes de restrição proteica materna In Utero / Transgenerational endocrine pancreatic adaptation in mice from maternal protein restriction in utero.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2011

RESUMO

Exposure of pregnant mice to a low-protein (LP) diet impairs endocrine pancreas development in their offspring and increases susceptibility to hypertension, diabetes and obesity in adulthood. There is evidence that this phenomenon may persist in subsequent generations. We sought to evaluate the effect of protein restriction on glucose metabolism and pancreatic morphometry in the F3 offspring of mice at birth and weaning. Virgin female Swiss mice (F0) were mated and received normal protein diet (19% protein - NP) or an isocaloric low protein diet (5% protein - LP) throughout pregnancy. Lactation and the rest of the experimental groups received NP diet. The male pups were named F1 (NP1 and LP1). F1 and F2 females were mated to produce F2 and F3 (NP2, LP2, NP3 and LP3). Weekly, pups were weighted and calculated the growth rate by allometry (log [body mass] = log a + b log [age]). The mice were sacrificed on days 1 and 21 of age, blood glucose was measured and the pancreas removed, weighed and analyzed by stereology and immunofluorescence, insulin was measured at 21 days. LP pups in the first generation (LP1) were smaller at birth, but had an accelerated growth and within 7 days catch-up growth with controls; LP2 offspring had higher body mass at birth and had a slower growth rate within 21 days; but there was no difference in body mass and growth rate in the F3 generation. The pancreatic mass decreased in LP1 through LP3 at birth but increased in LP2 at weaning. The islet volume density and diameter were smaller in all restricted groups at day 1 and 21, and LP1 had the lowest islet number; at birth, beta cell mass was smaller in LP1 through LP3 and remained low throughout suckling. At day 1 and 21, pups were normoglycemic, but were hypoinsulinemic at weaning. Thus, protein restriction in mice during pregnancy produces morphologic changes in pancreatic islets, suggesting that glucose homeostasis is maintained by an increased sensitivity to insulin during the early stages of life in offspring over three consecutive generations.

ASSUNTO(S)

beta cell glucose metabolism mice programação transgeracional restrição proteica materna célula beta metabolismo da glicose camundongos morfologia transgenerational programming protein restriction

ACESSO AO ARTIGO

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