Additional Restriction Endonuclease Cleavage Sites on the Bacteriophage P22 Genome
AUTOR(ES)
Casjens, Sherwood
RESUMO
We present complete restriction endonuclease cleavage site maps of the bacteriophage P22 chromosome for 16 enzymes with six base recognition sequences, thereby positioning 116 new sites on the chromosome. Twenty-four such restriction maps for P22 DNA, containing 162 sites, have now been completed, and three enzymes were found that did not cut P22 DNA. Our results are consistent with the ideas that ClaI does not cleave the methylated recognition sequence ATCGAmeT or AmeTCGAT and StuI does not cleave the methylated recognition sequence AGGCCmeT.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=256481Documentos Relacionados
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