Adenovirus sequences required for replication in vivo.
AUTOR(ES)
Wang, K
RESUMO
We have studied the in vivo replication properties of plasmids carrying deletion mutations within cloned adenovirus terminal sequences. Deletion mapping located the adenovirus DNA replication origin entirely within the first 67 bp of the adenovirus inverted terminal repeat. This region could be further subdivided into two functional domains: a minimal replication origin and an adjacent auxillary region which boosted the efficiency of replication by more than 100-fold. The minimal origin occupies the first 18 to 21 bp and includes sequences conserved between all adenovirus serotypes. The adjacent auxillary region extends past nucleotide 36 but not past nucleotide 67 and contains the binding site for nuclear factor I.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=321857Documentos Relacionados
- DNA sequences required for the in vitro replication of adenovirus DNA.
- The origin of adenovirus DNA replication: minimal DNA sequence requirement in vivo.
- Transcriptional targeting of replication-defective adenovirus transgene expression to smooth muscle cells in vivo.
- The adenovirus-2 EIIa early gene promoter: sequences required for efficient in vitro and in vivo transcription.
- Flanking sequences affect replication arrest at the Escherichia coli terminator TerB in vivo.