Adenylate Cyclase in Thymus-Derived and Bone Marrow-Derived Lymphocytes from Normal Donors and Patients with Chronic Lymphocytic Leukemia
AUTOR(ES)
Mendelsohn, John
RESUMO
Lymphocytes were purified from peripheral blood of normal donors and patients with chronic lymphocytic leukemia (CLL) by Ficoll-Hypaque centrifugation. Adenylate cyclase activity, expressed as picomoles [32P]cyclic AMP generated per milligram protein per minute, was 57±4 in normals and 26±4 in CLL patients. Enzyme activity, expressed as picomoles [32P]cyclic AMP generated per 106 lymphocytes per minute, was 2.09±0.19 for normal lymphocytes and 1.10±0.16 for CLL lymphocytes. The differences between normal and CLL peripheral lymphocytes are highly significant (P < 0.001) with either method of calculating activity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=372059Documentos Relacionados
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