Agar Block Technique for Identification of Mycoplasmas by Use of Fluorescent Antibody1
AUTOR(ES)
Baas, E. J.
RESUMO
A procedure for staining mycoplasmata colonies directly on agar blocks for examination by fluorescent microscopy is described. Areas of the agar surface appropriate for staining were demarcated by use of Lucite cylinders. Direct fluorescent-antibody staining was superior to indirect staining. The technique was very useful for determining whether cultures were mixed and for identification of mycoplasmas in either pure or mixed cultures.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=380513Documentos Relacionados
- IDENTIFICATION OF ERYSIPELOTHRIX INSIDIOSA WITH FLUORESCENT ANTIBODY1
- Differentiation of Cariogenic Streptococci by Fluorescent Antibody1
- IDENTIFICATION OF STREPTOCOCCI IN BACTERIAL MIXTURES AND CLINICAL SPECIMENS WITH FLUORESCENT ANTIBODY1
- Detection of Salmonella in Eggs and Egg Products With Fluorescent Antibody1
- Identification of Actinomyces israelii and Actinomyces naeslundii by Fluorescent-Antibody and Agar-Gel Diffusion Techniques1