Agglutinating and Bactericidal Properties of Fractions of Rabbit Anti-Vibrio cholerae Serum

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RESUMO

The major portion of the agglutinating and bactericidal activity of the sera of rabbits immunized with live Vibrio cholerae or with cholera vaccine was found in the γM fractions during the early stages of immunization. After 5 weeks or more, γG fractions accounted for more than half of the agglutinating activity. When late antibody was measured as the amount of protein precipitated by somatic antigens, nearly 3 times as much γG as γM was required for agglutination, and about 30 times as much γG as γM was required to kill 50% of a standard inoculum in the presence of complement. The ratio of vibriocidal to agglutinin titer of γG fractions at different stages of immunization was more variable than that of γM fractions. More complement was required for a vibriocidal effect by γG than by γM. Increasing the amount of complement decreased the amount of both γG and γM required to kill, but smaller amounts of γM required disproportionately larger amounts of complement. Less time was required by γM than by γG to kill 50% of the inoculum. Removal of the group-reactive antibody from anti-Ogawa serum and serum fractions by absorption with Inaba reduced the vibriocidal titer by more than one-half.

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