Alendronate of sodium, risedronate of sodium atorvastatin calcic and lovastatin in tibiais fractures in female rats with dexamethasone osteoporosis induced / Alendronato de sódio, risedronato sódico, atorvastatina cálcica e lovastatina na reparação de fraturas tibiais em ratas com osteoporose induzida pela dexametasona

Davilson Bragine Ferreira Júnior

This work consisted of a biological test with the objective to study the influences of the bifosfonates alendronate of sodium and sodic risedronate and of the statins calcic atorvastatine and lovasatatine in the recuperation of fractures of shin-bones of osteoporotics rats induced by the dexamethasone, in rats of the race Wistar, adults, weighing 250 20g. The process of the induction of the osteoporosis consisted of the administration of dexamethasone, of the dosis of 7,5 mg / kg of physical weight, for road IM, once weekly, during four weeks, of six animals of all the groups, with the exception of the animals that there appointed the control group (G1). The animals of the group 2 (G2), called osteoporotics, received only dexamethasone. After the period of induction of the osteoporosis, the animals of all the groups were subjected to the proceeding of shut fracture of the leftt shin-bones, carried out by a veterinary doctor. Immediately after the induction of the fractures, one began the treatments where the group 3 (G3) received alendronate of sodium in the dosis of 0,25mg/kg; the group 4 (G4) received risedronate of sodium in the dosis of 1 mg / kg; the group 5 (G5) that received calcic atorvastatine in the dosis of 1,2 mg / kg and the group 6 (G6) what it received lovastatine in the dosis of 2mg/kg. All the substances were administered by oral road, daily. In the period of 35 days, after the beginning of the treatments, samples of blood were collected for dosages of calcium, phosphorus, albumin and total proteins in the multiparametric equipment of biochemistry Alizé with kits of the mark BioMerieux, and phosphatase alkaline bone in the equipment of quimioluminescence Access Immunoassay System of Beckman Coulter with kits Ostase. After the sacrifice, there was collected the left shin-bone of each animal what, once fixed they were morfometric analysed, X- rayed and when it was prosecuted for getting the blades, which parliament Masson Tricrômic was colored, for hitological study in light microscopie. The right shin-bone was collected to subject to biomechanical tests. Alls measures of height, width and of the bone callus of the shin-bones they were carried out with help of a paquimeter. Besides, there were carried out gauging of the density of these bones, which took place through the relation of the values of weighing and dislocation of liquid in graduated container. The left shin- bones were X-rayed then in profile and the obtained image was subjected to an analysis of radiografic density through the program Image Tool he will be Windows 3.0, for tones of ash obtained of the image. The areas of the analyses were delimited in all the radiografic images of the shin-bones and only the selected areas were taken into account for analysis. Tests of flexing, in the right shin-bones they were carried out with the help of the appliance Instrom (Laboratory of Paper and Cellulose of the Department of Engineering Florestal/UFV). One proceeded the percentage calculation of the density trabecular bone, from the image amostral of trabecular bone, contained in the region subcondral, of each histological cut, in optical microscope equipped with digital camera (TCL-984 P), analysed in monitor of microcomputer of 14 inches, making use of the technique of histomorfometric of counting of points. The biological test was carried out according to delineation completely casualizado, with six treatments in six repetitions. The obtained results were subjected to the variance analysis and to the test F (p <0,05). Control the groups (G1 and G2) they were compared between you, through the test F. The treated groups (G3, G4, G5 and G6) were compared between you, through the test of Tukey to 5% of probability. The comparisons also were carried out between the treated groups and the controls G1 and G2, being that, for the same thing, the test of Dunnet was applied to 5 % of probability. The results did not show significant differences between the group control (G1) and the osteoporotic group (G2), as well as, between them and the treated groups (G3, G4, G5 and G6) and, also, between the groups treated between you, in all the periods, as for the values silken of calcium, phophorus and phosphatase alkaline bone. Through the morfometric and histomorfometric was possible the induction of the osteoporose noted with the glucorticoid, as well as the improvement in the bone density, density trabecular bone and thickness of the bone callus in all the treated groups when the group is prevailing treated with alendronate of sodium that presented better results. The results radiografics and biomechanical tests corroborate with the histomorfometric results. The biochemical results, principally phosphatase alkaline bone, what remained without significant differences in all the groups, when valued together with the morfometric, histomorfometri, radiografic and biomechanical test demonstrate what prevailed the properties antirreabsortives in relation the forming ones of bone in all the treated groups. In spite of the predominance of the properties antirreabsortives in all the treatments, the morfometric, histomorfometric, X-ray and biomechanical tests showed comparable results to the bone recuperation of the normal group, which demonstrates what same do not interfere negatively and which positively in the process of recuperation of fractures in osteoporotics animals. Is excepted, however, which subsequent studies of the dosages, interactions, biodisponibility, besides the toxicological effects of these pharmacological substances, make to themselves necessary.

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