Alloreactive cytolytic T-cell clones preferentially recognize conformational determinants on histocompatibility antigens: analysis with genetically engineered hybrid antigens.
AUTOR(ES)
Arnold, B
RESUMO
Hybrid genes were constructed for the localization of allodeterminants on murine class I antigens recognized by antibodies and cytolytic T lymphocytes. By using deletion subclones of the H-2Kd and H-2Kk genes, homologous regions were exchanged between the two alleles. The altered genes were introduced and expressed in mouse fibroblast and fibrosarcoma cells. Cells expressing hybrid antigens were analyzed with 29 monoclonal anti-H-2Kd and anti-H-2Kk antibodies and with 150 short-term alloreactive cytolytic T-cell clones. When only the first or only the second amino-terminal domain was exchanged, most T cells and 60% of the antibodies lost their reactivity to the H-2K antigen. No T-cell clone was directed against the third extracellular domain, whereas three antibodies could bind to this domain. This implies that nearly all determinants essential for a cytolytic T-cell response or for antibody binding lie on the two external domains and are conformational structures generated by the interaction of these two domains.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=391303Documentos Relacionados
- Bovine helper T cell clones recognize five distinct epitopes on Babesia bovis merozoite antigens.
- T-cell clones and T-cell receptors.
- Cell surface polypeptides of murine T-cell clones expressing cytolytic or amplifier activity.
- Cytolytic T-cell clones against an autologous human melanoma: specificity study and definition of three antigens by immunoselection.
- T-cell modulation of the antibody response to bacterial polysaccharide antigens.