Alterations in Chloroplast Thylakoids during an in Vitro Freeze-Thaw Cycle 1

AUTOR(ES)
RESUMO

Plastocyanin and chloroplast coupling factor 1 (CF1) are released from spinach (Spinacia oleracea L.) thylakoids during a slow freezethaw cycle. CF1 addition increases the proton uptake of thylakoids previously frozen in sucrose concentrations of 15 mm to 100 mm. Addition of CF1 and plastocyanin restores the proton uptake of thylakoids frozen in 100 mm sucrose. Plastocyanin and CF1 release is a manifestation, not the cause, of freeze-thaw damage.

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