Altered Diaphorase Activity in Optochin-resistant Pneumococci1
AUTOR(ES)
Kreger, Arnold S.
RESUMO
Methylene blue (MB) reductase activities of washed cell suspensions and of cell-free extracts prepared from optochin-resistant mutant pneumococci were two times greater than and four to eight times more resistant to optochin inhibition than those of similar preparations from the optochin-sensitive parent strains. With whole cells, optochin hydrochloride was approximately four times more potent than quinine hydrochloride in inhibiting MB reductase activity. However, with cell-free extracts, both drugs had similar inhibitory activities. Glucose uptake was not affected by optochin hydrochloride, and both optochin-sensitive and optochin-resistant pneumococci had similar glucose uptake patterns. Diaphorase activities of cell-free extracts prepared from optochin-resistant pneumococci were two times greater than and four to eight times more resistant to optochin inhibition than those of cell-free extracts prepared from the optochin-sensitive parent strains. Flavin concentrations of cell-free extracts prepared from optochin-sensitive and optochin-resistant strains were similar.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=252413Documentos Relacionados
- Comparison of Five Genotypic Techniques for Identification of Optochin-Resistant Pneumococcus-Like Isolates
- Dehydrogenase Characteristics of the Pneumococci1
- RECIPROCAL CAPSULAR TRANSFORMATIONS OF PNEUMOCOCCI1
- EFFECTS OF ENZYMATIC DEOXYRIBONUCLEIC ACID DIGESTS ON POPULATION CHANGES AND DEOXYRIBONUCLEIC ACID SYNTHESIS OF PNEUMOCOCCI1
- QUANTITATIVE DETERMINATION, IN TYPE-SPECIFIC ANTISERA TO HEMOPHILUS INFLUENZAE, OF THE ANTIBODY THAT CROSS-REACTS WITH ENCAPSULATED PNEUMOCOCCI1