Amikacin resistance mediated by multiresistance transposon Tn2424.

AUTOR(ES)

Meyer, J F

RESUMO

Tn2424, a multiresistance transposon 25 kilobases long, was isolated from IncFII plasmid NR79. Tn2424 transposed resistance to sulfonamides, streptomycin and spectinomycin, mercuric chloride, chloramphenicol, and amikacin with a frequency of 6 X 10(-5). Resistance to amikacin was mediated by a 6'-N-acetyltransferase, which conferred higher levels of resistance in Pseudomonas aeruginosa than in Escherichia coli. A restriction analysis and cloning experiments resulted in a physical and functional map of Tn2424. Comparison by a heteroduplex technique revealed that Tn2424 includes the total sequence of Tn21 and two additional DNA fragments that are 1.8 and 4 kilobases long.

Documentos Relacionados

• Tn1331, a novel multiresistance transposon encoding resistance to amikacin and ampicillin in Klebsiella pneumoniae.
• Transposon-mediated amikacin resistance in Klebsiella pneumoniae.
• Host-dependent transposon Tn5-mediated streptomycin resistance.
• Bleomycin increases amikacin and streptomycin resistance in Escherichia coli harboring transposon Tn5.
• Characterization of transposon Tn1528, which confers amikacin resistance by synthesis of aminoglycoside 3'-O-phosphotransferase type VI.