Amniotic membrane as an option for treatment of acute Achilles tendon injury in rats
AUTOR(ES)
Nicodemo, Mariana de Castro, Neves, Lia Renó das, Aguiar, Josafá Carvalho, Brito, Flaviane de Souza, Ferreira, Isabelle, Sant'Anna, Luciana Barros, Raniero, Leandro José, Martins, Rodrigo Álvaro Lopes, Barja, Paulo Roxo, Arisawa, Emilia Angela Lo Schiavo
FONTE
Acta Cir. Bras.
DATA DE PUBLICAÇÃO
2017-02
RESUMO
Abstract Purpose: To evaluate the effect of human amniotic membrane (hAM) fragment on inflammatory response, proliferation of fibroblast and organization of collagen fibers in injured tendon. Methods: Sixty rats were divided into 3 groups: C - surgical procedures without tendon lesion and with simulation of hAM application; I - surgical procedures, tendon injury and simulation of hAM application; T - surgical procedures, tendon injury and hAM application. These groups were subdivided into four experimental times (3, 7, 14 and 28 days). The samples underwent histological analysis and ATR-FTIR spectroscopy. Results: Histological analysis at 14 days, the T group showed collagen fibers with better alignment. At 28 days, the I group presented the characteristics described for the T group at 14 days, while this group presented aspects of a mature connective tissue. FT-IR analysis showed a clear distinction among the three groups at all experimental times and groups T and I presented more similarities to each other than to group C. Conclusion: Acute injury of tendon treated with human amniotic membrane fragment showed a faster healing process, reduction in inflammatory response, intense proliferation of fibroblasts and organization of collagen fibers.
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