Amplification mediated by polyomavirus large T antigen defective in replication.
AUTOR(ES)
St-Onge, L
RESUMO
The polyomavirus large T antigen promotes homologous recombination at high rates when expressed in rat cells carrying the viral replication origin and two repeats of viral DNA sequences stably integrated into the cellular genome. Recombination consists of both reciprocal and nonreciprocal events and is promoted by mutants defective in the initiation of viral DNA synthesis (L. St-Onge, L. Bouchard, and M. Bastin, J. Virol. 67:1788-1795, 1993). We have extended our studies to a rat cell line undergoing amplification of the viral insert. We show that large T antigen promotes amplification independently of its replicative function but that its origin-specific DNA binding activity is not sufficient to promote homologous recombination.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=237891Documentos Relacionados
- High-frequency recombination mediated by polyomavirus large T antigen defective in replication.
- Elements of the polyomavirus replication origin required for homologous recombination mediated by large T antigen.
- Enhancer-mediated role for polyomavirus middle T/small T in DNA replication.
- Constitutive expression of simian virus 40 large T antigen in monkey cells activates their capacity to support polyomavirus replication.
- AP1 enhances polyomavirus DNA replication by promoting T-antigen-mediated unwinding of DNA.