An 'elaborated' pseudoknot is required for high frequency frameshifting during translation of HCV 229E polymerase mRNA.
AUTOR(ES)
Herold, J
RESUMO
The RNA polymerase gene (gene 1) of the human coronavirus 229E is approximately 20 kb in length and is located at the 5' end of the positive-strand genomic RNA. The coding sequence of gene 1 is divided into two large open reading frames, ORF1a and ORF1b, that overlap by 43 nucleotides. In the region of the ORF1a/ORF1b overlap, the genomic RNA displays two elements that are known to mediate (-1) ribosomal frameshifting. These are the slippery sequence, UUUAAAC, and a 3' pseudoknot structure. By introducing site-specific mutations into synthetic mRNAs, we have analysed the predicted structure of the HCV 229E pseudoknot and shown that besides the well-known stem structures, S1 and S2, a third stem structure, S3, is required for a high frequency of frameshifting. The requirement for an S3 stem is independent of the length of loop 2.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=310462Documentos Relacionados
- Nucleotide sequence of the human coronavirus HCV 229E mRNA 4 and mRNA 5 unique regions.
- A uridine-rich sequence required for translation of prokaryotic mRNA.
- Ribosome pausing and stacking during translation of a eukaryotic mRNA.
- Ribosomal frameshifting during translation of measles virus P protein mRNA is capable of directing synthesis of a unique protein.
- A nuclear gene in maize required for the translation of the chloroplast atpB/E mRNA.
