An erythroid-specific, developmental-stage-independent enhancer far upstream of the human "beta-like globin" genes.
AUTOR(ES)
Tuan, D Y
RESUMO
We have identified an erythroid-specific enhancer element far upstream of the human "beta-like globin" genes, at 10.2-11.0 kilobases 5' of the embryonic epsilon-globin gene, and thus at 53-54 kilobases 5' of the adult beta-globin gene. It is capable of enhancing the expression of a cis-linked test gene by up to 300-fold. This enhancer element is apparently developmental-stage-independent, as it is functional at the embryonic and the adult developmental stages in erythroid cells that are expressing the respective beta-like globin genes. The enhancer and globin promoter sequences work in synergy and are capable of conferring on a cis-linked gene the high transcriptional efficiency (enhancer function), erythroid specificity (enhancer and promoter functions), and developmental-stage specificity (promoter function) that are characteristic of the in vivo transcription of the beta-like globin genes in erythroid cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=286955Documentos Relacionados
- Protein-DNA interactions in vivo of an erythroid-specific, human beta-globin locus enhancer.
- Erythroid-specific expression of human beta-globin genes in transgenic mice.
- Tissue-specific DNA methylation in a cluster of rabbit beta-like globin genes.
- Erythroid-specific nuclease-hypersensitive sites flanking the human beta-globin domain.
- Developmental regulation of the human embryonic beta-like globin gene is mediated by synergistic interactions among multiple tissue- and stage-specific elements.