An Escherichia coli Mutant with an Altered Elongation Factor Tu

AUTOR(ES)

Lupker, J. H.

RESUMO

A thermosensitive mutant of Escherichia coli has been isolated that is unable to replicate the bacteriophage MS2 at 42° but permits phage production at 37°. Thermal inactivation studies of the supernatant enzymes show that this mutant contains a factor essential for the polymerization of phenylalanine from phenylalanyl-tRNA that at 50° is more rapidly inactivated than the corresponding wild-type factor. The elongation factor Tu (EF-Tu) was isolated and purified to apparent homogeneity as the EF-Tu·GDP complex, both from mutant and wild-type cells.

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