An internal 5'-noncoding region required for translation of poliovirus RNA in vitro.

AUTOR(ES)

Bienkowska-Szewczyk, K

RESUMO

A truncated poliovirus RNA that contains the entire 5'-noncoding region as well as some capsid protein-coding sequences was produced from cloned cDNA inserted into an SP6 transcription vector and subsequently was translated in a mixed rabbit reticulocyte-HeLa cell lysate. Deletions or modifications of regions of the 5'-noncoding sequences had significant effects upon the efficiency of translation. The presence of a 60-nucleotide sequence located at positions 567 to 627 appeared to be essential for active ribosome binding and translation of this uncapped RNA.

Documentos Relacionados

• Internal translation initiation on poliovirus RNA: further characterization of La function in poliovirus translation in vitro.
• The 5' nontranslated region of hepatitis A virus RNA: secondary structure and elements required for translation in vitro.
• trans complementation of cap-independent translation directed by poliovirus 5' noncoding region deletion mutants: evidence for RNA-RNA interactions.
• Attenuation stem-loop lesions in the 5' noncoding region of poliovirus RNA: neuronal cell-specific translation defects.
• Cap-independent translation of poliovirus mRNA is conferred by sequence elements within the 5' noncoding region.