An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues
AUTOR(ES)
Liu, Chang-Gong
FONTE
National Academy of Sciences
RESUMO
MicroRNAs (miRNAs) are a class of small noncoding RNA genes recently found to be abnormally expressed in several types of cancer. Here, we describe a recently developed methodology for miRNA gene expression profiling based on the development of a microchip containing oligonucleotides corresponding to 245 miRNAs from human and mouse genomes. We used these microarrays to obtain highly reproducible results that revealed tissue-specific miRNA expression signatures, data that were confirmed by assessment of expression by Northern blots, real-time RT-PCR, and literature search. The microchip oligolibrary can be expanded to include an increasing number of miRNAs discovered in various species and is useful for the analysis of normal and disease states.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=470744Documentos Relacionados
- MicroRNA expression detected by oligonucleotide microarrays: System establishment and expression profiling in human tissues
- Genome-wide expression profiling of human blood reveals biomarkers for Huntington's disease
- Genome-wide expression profiling in Escherichia coli K-12.
- Expression Profiling by iAFLP: A PCR-Based Method for Genome-Wide Gene Expression Profiling
- A Genome-Wide Survey of Human Pseudogenes
