An ultrastructural study of implantation in the golden hamster. I. Loss of the zona pellucida and initial attachment to the uterine epithelium.

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RESUMO

Forty five blastocysts from 14 golden hamsters were examined in situ with light and electron microscopy between 3 and 4 days of pregnancy (post-ovulation). At 3 days blastocysts remained free within the uterine lumen with their zonae pellucidae intact. At various intervals between 3 days and 3 days 6 hours a gradual thinning of the zona occurred, indicating the possible presence of a lytic factor which aids in the removal of the zona pellucida. Blastocysts at 3 days 8 hours had lost their zonae pellucidae, and by 3 1/2 days they were completely enclosed by the uterine pithelium. By 4 days a more intimate association between the juxtaposed trophoblast and uterine epithelium occurred, leaving intermittent deposits of electron-dense material between the adjoining plasma membranes.

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