An upstream open reading frame impedes translation of the huntingtin gene
AUTOR(ES)
Lee, Joseph
FONTE
Oxford University Press
RESUMO
Expansion of a CAG tract within the huntingtin gene, leading to the production of a protein with an expanded polyglutamine tract, is responsible for Huntington’s disease. We show here that the 5′ untranslated region (UTR) of the huntingtin gene plays an important role in controlling the synthesis of huntingtin. In particular, the 5′ UTR contains an upstream open reading frame (uORF) encoding a 21 amino acid peptide. We demonstrate that the presence of this uORF negatively influences expression from the huntingtin mRNA. Our results suggest a role for the uORF in limiting ribosomal access to downstream initiation sites. Mechanisms involving the post-transcriptional regulation of huntingtin are not well understood, and this may be an important way of regulating huntingtin protein levels.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=137975Documentos Relacionados
- An open reading frame upstream from the nifH gene of Klebsiella pneumoniae.
- Role of an upstream open reading frame in the translation of polycistronic mRNAs in plant cells.
- A Conserved Upstream Open Reading Frame Mediates Sucrose-Induced Repression of TranslationW⃞
- Nucleotide sequence of ORF2: an open reading frame upstream of the tRNA ligase gene.
- Translation of the mRNA for the sporulation gene spoIIID of Bacillus subtilis is dependent upon translation of a small upstream open reading frame.