Anaerobic C1 Metabolism of the O-Methyl-14C-Labeled Substituent of Vanillate
AUTOR(ES)
Frazer, Anne Cornish
RESUMO
The O-methyl substituents of aromatic compounds constitute a C1 growth substrate for a number of taxonomically diverse anaerobic acetogens. In this study, strain TH-001, an O-demethylating obligate anaerobe, was chosen to represent this physiological group, and the carbon flow when cells were grown on O-methyl substituents as a C1 substrate was determined by 14C radiotracer techniques. O-[methyl-14C]vanillate (4-hydroxy-3-methoxy-benzoate) was used as the labeled C1 substrate. The data showed that for every O-methyl carbon converted to [14C]acetate, two were oxidized to 14CO2. Quantitation of the carbon recovered in the two products, acetate and CO2, indicated that acetate was formed in part by the fixation of unlabeled CO2. The specific activity of 14C in acetate was 70% of that in the O-methyl substrate, suggesting that only one carbon of acetate was derived from the O-methyl group. Thus, it is postulated that the carboxyl carbon of the product acetate is derived from CO2 and the methyl carbon is derived from the O-methyl substituent of vanillate. The metabolism of O-[methyl-14C]vanillate by strain TH-001 can be described as follows: 314CH3OC7H5O3 + CO2 + 4H2O → 14CH3COOH + 214CO2 + 10H+ + 10e- + 3HOC7H5O3.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=238819Documentos Relacionados
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