Análise citopatológica de células de inseto infectadas com baculovírus mutantes

AUTOR(ES)

Cláudia Natércia Lima Acácio

DATA DE PUBLICAÇÃO

2007

RESUMO

Advances in cell culture techniques allowed the isolation and precise characterization of baculoviruses mutants. The majority of these mutants is derived from the virus AcMNPV, mainly due to its facility of propagation and stability in cell culture. During the purification routine of a recombinant baculovirus, two mutants derived from AcMNPV, vSynlitx1B12P (MutPolh) and vSynlitx1G4 (MutApo) were isolated (unpublished). The insect cell by the MutPolh did not result in polyhedra production and differed from wild-type infection by the presence of proteinaceous masses dispersed in the cytoplasm. To explain this phenotype, the virus MutPolh was previously investigated and molecular analysis revealed a point mutation in the polyhedrin gene. This gene is highly conserved among baculoviruses and changes in its nucleotide sequence may lead to mutant polyhedra, morphologically different from the wild-type. The other mutant virus, MutApo, was show to induce quick and massive cell death in infected cells. This mutant was also investigated and was found a DNA insertion interrupting an inhibitor of apoptosis gene (p35). In this work, the cytopathologic effects induced by these two mutants in Sf-21 and Tn-5B insect cell lines were analyzed. Structural and ultrastructural studies confirmed the findings of the molecular analysis previously done for these mutants. The observation of MutPolh-infected cells revealed that the proteinaceous aggregates found in the cytoplasm were, actually, accumulation of uncrystalized polyhedrin. Observations of cells infected by the MutApo mutant also confirmed previous information, showing that this mutant induces characteristic apoptosis events in these insect cell lines

ASSUNTO(S)

apoptosis baculovírus apoptose biologia molecular poliedrina polyhedrin baculovirus

Documentos Relacionados

• Estudos morfologicos do processo infeccioso e ocorrencia de apoptose em cultura de celulas de inseto infectadas por baculovirus mutantes
• Desenvolvimento de meio de cultura para celulas de inseto e avaliação do potencial de replicação de baculovirus
• Expressão de proteínas do vírus da dengue em células de inseto utilizando o sistema baculovírus de expressão
• Geração e acumulo de mutantes com poucos poliedros após passagem serial do baculovirus anticarsia (AgMNPV) em cultura de células.
• Replicação de um baculovírus recombinante contendo o gene rolA de Agrobacterium rhizogenes em cultura de células de inseto.