ANÁLISE DA VARIAÇÃO DE 11 MARCADORES DE MICROSSATÉLITES DE Plasmodium falciparum EM PACIENTES SINTOMÁTICOS E ASSINTOMÁTICOS DE UMA COMUNIDADE EM ZONA ENDEMICA DE MALÁRIA DE PORTO VELHO-RO / ANÁLISE DA VARIAÇÃO DE 11 MARCADORES DE MICROSSATÉLITES DE Plasmodium falciparum EM PACIENTES SINTOMÁTICOS E ASSINTOMÁTICOS DE UMA COMUNIDADE EM ZONA ENDEMICA DE MALÁRIA DE PORTO VELHO-RO

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

In the present study, genotypes of Plasmodium falciparum parasites obtained from residents of different suburbs of Porto Velho were compared by means of microsatellite analysis. Fourty-four samples, divided in five groups were analyzed: two groups of samples from non-symptomatic infections (Candelaria, taken at different timepoints) and three groups of samples from symptomatic patients (from Candelaria, Bate-Estaca and CEPEM) were analyzed using eleven different microsatellite markers. For each distinct group, the diversity index, the fixation index (Fst-value) and the percentage of mixed (polyclonal) infections. All groups presented multiple infections to a similar degree. As expected, the group of samples from CEPEM showed the highest degree of heterozigosity, since samples were from patients of several different locations. There was no significant difference in heterozygosity between samples from asymptomatic and symptomatic infections of the same location. The largest genetic differentiation was observed between Candelária (asymptomatics) and Bate Estaca (symptomatics) samples, in spite of the geographical proximity of the two locations. This study confirms that the rather similar circulating Plasmodium falciparum genotypes, determined by microsatellite analysis, can either both cause symptomatic or asymptomatic infections and that the reason for this probably lies in the differential expression of virulence associated genes.

ASSUNTO(S)

marcadores de microssatÉlites pacientes sintomÁticos biologia geral marcadores de microssatÉlites pacientes sintomÁticos

ACESSO AO ARTIGO

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