Analise eletroforetica de proteinas de membrana de Pseudomonas avenae e Pseudomonas rubrilineans patogenicas a gramineas

AUTOR(ES)
DATA DE PUBLICAÇÃO

1991

RESUMO

Electrophoretical analysis (SDS-PAGE) of whole cell proteins and membrane fraction proteins were used to compare Pseudomonas avenae (MANNS, 1909) with Pseudomonas rubrilineans (LEE et al, 1925) STAPP, 1928) strains from different hosts. Antibiotic sensitivity and bacteriocin production tests were also analised. The electrophoretical analysis of the membrane -fraction protein showed differences in low molecular weight protein profiles, between MW 24.000 and 14.000. Strains which were grouped by numerical analysis of protein profiles were similar to those based only on low molecular weight -protein profiles. It was verified a possible relationship between protein profiles and the original host of strains. No differences were observed between P. avenae and P. rubrilineans strains when the whole cell protein samples were compared. Moreover, the differences observed in low molecular weight proteins of the-membrane fractions were not found in total protein profiles. P. avenae and P. rubrilineans did not differ on sensit ivity to ampicilin, tetracycline, streptomycin, chloramphenicol and kanamycin, in different concentrations. The results of this work suggest sinonymy of P. avenae and P. rubrilineans cultures, however, the analysis of membrane proteins have shown distinct strain groups from corn and sugarcane. Also, some P. avenae and P. rubrilineans strains showed distinct characteristics in bacteriocin production and host assay tests. The use of other techniques, such as restriction DNA profile, to improve the understanding of the taxonomic position of these bacteria is discussed

ASSUNTO(S)

plantas forrageiras ecologia vegetal fisiologia vegetal

ACESSO AO ARTIGO

Documentos Relacionados