Analysis and comparison of the internal promoter, pE, of the ilvGMEDA operons from Escherichia coli K-12 and Salmonella typhimurium.

AUTOR(ES)

Lopes, J M

RESUMO

It was previously determined that the distal portion of the ilvGMEDA operon was expressed despite the insertion of transposons into ilvG and ilvE. This observation suggested the existence of internal promoters upstream of ilvE (pE) and ilvD (pD). The internal promoter pE, responsible for part of ilvEDA expression, has been analyzed both in vivo and in vitro. Our results indicate that: pE exists in both E. coli K-12 and S. typhimurium; pE is located in the distal end of the ilvM coding sequence; the pE sequence is highly conserved in the two bacteria; the amino acid sequence of the ilvM gene product is 93% homologous between the two bacteria; transcription from pE can be demonstrated both in vivo and in vitro; the efficiency of pE is essentially equivalent in the two bacteria.

Documentos Relacionados

• Examination of the internal promoter, PE, in the ilvGMEDA operon of E. coli K-12.
• The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12
• The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12
• Specificity of Attenuation Control in the ilvGMEDA Operon of Escherichia coli K-12
• The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12.