Analysis of ancient DNA from coprolites: a perspective with random amplified polymorphic DNA-polymerase chain reaction approach
AUTOR(ES)
Iñiguez, Alena M, Araújo, Adauto, Ferreira, Luiz Fernando, Vicente, Ana Carolina P
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2003-01
RESUMO
The aim of this work was to determine approaches that would improve the quality of ancient DNA (aDNA) present in coprolites to enhance the possibility of success in retrieving specific sequence targets. We worked with coprolites from South American archaeological sites in Brazil and Chile dating up to 7,000 years ago. Using established protocols for aDNA extraction we obtained samples showing high degradation as usually happens with this kind of material. The reconstructive polymerization pretreatment was essential to overcome the DNA degradation and the serial dilutions helped with to prevent polymerase chain reaction (PCR) inhibitors. Moreover, the random amplified polymorphic DNA-PCR has been shown to be a reliable technique for further experiments to recover specific aDNA sequences.
Documentos Relacionados
- A population genetics study of Anopheles darlingi (Diptera: Culicidae) from Colombia based on random amplified polymorphic DNA-polymerase chain reaction and amplified fragment lenght polymorphism markers
- DNA-polymerase inhibitors. Rifamycin derivatives.
- Genetic analysis with random amplified polymorphic DNA markers.
- Application of polymerase chain reaction to fingerprinting Aspergillus fumigatus by random amplification of polymorphic DNA.
- Typing of group A streptococci by random amplified polymorphic DNA analysis.