Analysis of immunoglobulin heavy chain V-region genes belonging to the V NP-gene family.
AUTOR(ES)
Blankenstein, T
RESUMO
A method was devised to clone immunoglobulin VH-region genes located on selected restriction fragments from genomic DNA directly into M13 vectors for subsequent nucleotide sequence analysis. Ten recombinant M13 clones representing four so far unknown VH-region genes of the VNP-gene family have been analysed. Sequence comparison shows that these genes are closely related to other VH-genes of the VNP gene family. One of the VH-genes exhibits a so far unobserved unusual length of 100 2/3 codons and appears to be functional. Analysis of the variation of the isolated VH-genes suggests that framework and complementarity determining regions are exposed to separate types of selective pressures.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=320124Documentos Relacionados
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