Analysis of Mycoplasma hyorhinis genome by use of restriction endonucleases and by electron microscopy.
AUTOR(ES)
Darai, G
RESUMO
The chromosome of Mycoplasma hyorhinis was analyzed by using different restriction endonucleases and electron microscopy. It was found that restriction enzymes BstEII, XhoI, and SacI are the enzymes of choice for analysis and characterization of M. hyorhinis. The bands resulting from digestion of M. hyorhinis DNA with BstEII had apparent molecular weights ranging from 1.2 X 10(6) to 75 X 10(6). The apparent total molecular weight of DNA was calculated from the molecular weights of the individual bands and found to be 251 X 10(6). Electron microscopic contour length measurements of the largest DNA fragments verified the molecular weight values calculated from gel analysis. Electron microscopic contour length measurements of intact DNA of M. hyorhinis revealed a molecular weight of 5.4 +/- 5 X 10(8). The discrepancy between the values of molecular weight of M. hyorhinis DNA as determined by restriction enzyme analysis and contour length measurement is based on the fact that some of the DNA fragments which migrate as an apparent single band in the agarose gel really are double or multiple DNA fragments.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=216431Documentos Relacionados
- Analysis of the Spodoptera frugiperda Nuclear Polyhedrosis Virus Genome by Restriction Endonucleases and Electron Microscopy
- Temporal analysis of the developing Chlamydia psittaci inclusion by use of fluorescence and electron microscopy.
- Analysis of oncornavirus RNA subunits by electron microscopy.
- Mycoplasma pneumoniae in hamster tracheal organ culture studied by scanning electron microscopy.
- Circular DNA of a yeast episome with two inverted repeats: structural analysis by a restriction enzyme and electron microscopy.