Analysis of Simian Virus 40-induced Transformation of Hamster Kidney Tissue In Vitro: V. Variability of Virus Recovery from Cell Clones Inducible with Mitomycin C and Cell Fusion

AUTOR(ES)

Burns, William H.

RESUMO

Clones of simian virus 40-transformed hamster kidney cells from which infectious virus could be recovered and clones which did not yield virus by the overlay technique were subjected to chemical (mitomycin C) induction and to co-cultivation and fusion studies with indicator cells. The results of these studies indicate that the complete viral genome is present in all clones tested but that considerable heterogeneity with respect to inducibility exists among the clones. It is suggested that differences either in the number of viral genomes per transformed cell or in the status of the viral genome in transformed cells exist among the various clones. Furthermore, the inducibility of the clones may be correlated with their malignant potential.

Documentos Relacionados

• Analysis of Simian Virus 40-Induced Transformation of Hamster Kidney Tissue in Vitro VIII. Induction of Infectious Simian Virus 40 from Virogenic Transformed Hamster Cells by Amino Acid Deprivation or Cycloheximide Treatment
• AN ANALYSIS OF SV 40-INDUCED TRANSFORMATION OF HAMSTER KIDNEY TISSUE IN VITRO, I. GENERAL CHARACTERISTICS
• Simian virus 40-Chinese hamster kidney cell interaction. V. Cooperative effect of 5-iodo-2'-deoxyuridine and mitomycin C in the enhancement of virus replication in infected cells.
• Purification of T antigen from nuclei of simian virus 40-induced hamster tumors.
• AN ANALYSIS OF SV40-INDUCED TRANSFORMATION OF HAMSTER KIDNEY TISSUE IN VITRO, II. STUDIES OF THREE CLONES DERIVED FROM A CONTINUOUS LINE OF TRANSFORMED CELLS