Analysis of Small Human Proteins Reveals the Translation of Upstream Open Reading Frames of mRNAs
AUTOR(ES)
Oyama, Masaaki
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
To find novel short coding sequences from accumulated full-length cDNA sequences, proteomic analysis of small proteins expressed in human leukemia K562 cells was performed using high-resolution nanoflow liquid chromatography coupled with electrospray ionization tandem mass spectrometry. Our analysis led to the identification of 54 proteins not more than 100 amino acids in length, including four novel ones. These novel short coding sequences were all located upstream of the longest open reading frame (ORF) of the corresponding cDNA. Our findings indicate that the translation of short ORFs occurs in vivo whether or not there exists a longer coding region in the downstream of the mRNA. This investigation provides the first direct evidence of translation of upstream ORFs in human cells, which could greatly change the current outline of the human proteome.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=528919Documentos Relacionados
- Role of an upstream open reading frame in the translation of polycistronic mRNAs in plant cells.
- Powdery Mildew-Induced Mla mRNAs Are Alternatively Spliced and Contain Multiple Upstream Open Reading Frames1
- Upstream Open Reading Frames as Regulators of mRNA Translation
- Efficiency of reinitiation of translation on human immunodeficiency virus type 1 mRNAs is determined by the length of the upstream open reading frame and by intercistronic distance.
- A translation-attenuating intraleader open reading frame is selected on coronavirus mRNAs during persistent infection.
