Analysis of the Function of a Putative 2,3-Diphosphoglyceric Acid-Dependent Phosphoglycerate Mutase from Bacillus subtilis

AUTOR(ES)

Pearson, Claire L.

FONTE

American Society for Microbiology

RESUMO

A Bacillus subtilis gene termed yhfR encodes the only B. subtilis protein with significant sequence similarity to 2,3-diphosphoglycerate-dependent phosphoglycerate mutases (dPGM). This gene is expressed at a low level during growth and sporulation, but deletion of yhfR had no effect on growth, sporulation, or spore germination and outgrowth. YhfR was expressed in and partially purified from Escherichia coli but had little if any PGM activity and gave no detectable PGM activity in B. subtilis. These data indicate that B. subtilis does not require YhfR and most likely does not require a dPGM.

Documentos Relacionados

• Possible diurnal variation in 2,3-diphosphoglyceric acid.
• Three deoxyribonucleic acid-dependent adenosine triphosphatases from Bacillus subtilis.
• Mechanism of action of thyroid hormones on erythrocyte 2,3-diphosphoglyceric acid synthesis
• Purification and properties of the manganese-dependent phosphoglycerate mutase of Bacillus subtilis.
• Nuclear Deoxyribonucleic Acid-Dependent Ribonucleic Acid Polymerases from Saccharomyces cerevisiae