Analysis of transcription termination signals in the nin region of bacteriophage lambda: the roc deletion.

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RESUMO

Deletions in the region, nin, between the P and Q genes of phage lambda remove a portion of the phage genome that includes signals for termination of transcription. These deletions were selected because they permit growth of lambda derivatives defective in the N-mediated transcription antitermination system; i.e., the deletions confer N independence (nin). Thus nin phages (e.g., lambda nin5) grow in most Escherichia coli nus mutants. The nus genes encode functions necessary for N action. We report the isolation of a deletion in the nin region delta roc that confers a partially N-independent phenotype; lambda derivatives with delta roc can grow under normally nonpermissive conditions, 32 degrees C, in a host with the rpoB-nusC60 mutation. The roc deletion also partially suppresses the inhibitory effects of other nus mutations at higher temperatures. Delta roc, which extends from base pairs 41883 to 43825, overlaps the nin5 deletion, which extend from base pairs 40501 to 43306. Unlike the nin5 deletion, the sequences deleted by delta roc do not include a stem-loop structure, tR2, previously shown to have terminator activity. Using promoter and terminator testor vectors, we demonstrate that a 2,400-base-pair fragment that includes the wild-type roc region but excludes tR2 has terminator activity. Thus, delta roc permits a functional division of the transcription termination signals in the nin region.

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