Analysis of unintegrated avian RNA tumor virus double-stranded DNA intermediates.
AUTOR(ES)
Hsu, T W
RESUMO
Previous studies by Guntaka et al. have shown that the unintegrated DNA intermediates of avian RNA tumor virus replication can be readily isolated from cultures of the quail tumor line QT-6 at 1 day after infection. The intermediates include double-stranded linear and covalently closed circular DNA species. Using the analysis procedure of Southern together with previously obtained information regarding the sites of action of certain restriction endonucleases on avian sarcoma virus DNA, we have further characterized the viral DNA intermediates. Evidence is presented that, relative to the RNA genome, most of the linear species possess a direct terminal sequence redundancy equivalent to 0.5 X 10(6) +/- 0.3 X 10(6) daltons of double-stranded DNA. Some of the circular forms also possess a sequence redundancy of 0.21 X 10(6) +/- 0.03 X 10(6) daltons.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=525805Documentos Relacionados
- Specific site of action for single-strand specific nuclease on the double-stranded circular DNA intermediates of an avian RNA tumor virus.
- Murine leukemia virus: detection of unintegrated double-stranded DNA forms of the provirus.
- Molecular cloning of double-stranded RNA virus genomes.
- A deletion mutant of L-A double-stranded RNA replicates like M1 double-stranded RNA.
- Packaging in a yeast double-stranded RNA virus.
