Antigenic determinant of the Lancefield group H antigen of Streptococcus sanguis.
AUTOR(ES)
Rosan, B
RESUMO
Previous studies indicated that the teichoic acid isolated from strains of Streptococcus sanguis was group specific and defined the Lancefield group H streptococci. To determine the specific antigenic determinants, the antigen was extracted from a group H streptococcus (ATCC 903) by the phenol-water method and purified by column chromatography. The isolated antigen had a glycerol/phosphate/glucose molar ratio of 1:0.9:0.3; the lipid concentration was 7.6% of its dry weight. No nucleic acids were detected, and amino acids constituted approximately 2% of the dry weight. The minimum concentration of antigen required to sensitize erythrocytes for hemagglutination with a 1:1,000 dilution of either group H antiserum or antiteichoic acid serum was 0.02 microgram/ml. Hemagglutination inhibition studies suggested that the major antigenic determinant consisted of an alpha-glucose linked to the glycerol phosphate backbone.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=347838Documentos Relacionados
- Recombination-deficient Streptococcus sanguis.
- Sanguicin, a bacteriocin of oral Streptococcus sanguis.
- Specific coaggregation and the cell wall of Streptococcus sanguis.
- Analysis of the immunoglobulin A protease gene of Streptococcus sanguis.
- Isolation and analysis of sacculi from Streptococcus sanguis.