Antiproliferative effects of antisense oligonucleotides directed to the RNA of c-myc oncogene.
AUTOR(ES)
Degols, G
RESUMO
Several groups have reported the use of antisense oligonucleotides to inhibit c-myc gene expression and study its biological role. However high concentrations of free oligonucleotides were generally needed. To lower their concentration and stabilize the antisense effect against c-myc, oligonucleotides were covalently linked to poly(L-lysine) and administered in ternary complexes formed with heparin (100 micrograms/ml). A sequence specific growth inhibition was observed at concentrations lower than 1 microM, while oligonucleotide-poly(L-lysine) conjugates alone were inefficient. Similar results occurred with other polyanionic compounds. Inhibition of proliferation was correlated to a reduction of c-myc protein and to a transient decrease in c-myc mRNA level. However, implication of RNase H in this process could not be demonstrated.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=333736Documentos Relacionados
- Analysis of polyadenylation site usage of the c-myc oncogene.
- Immortalization of mouse neural precursor cells by the c-myc oncogene.
- Posttranscriptional regulation of cellular gene expression by the c-myc oncogene.
- The antiproliferative activity of c-myb and c-myc antisense oligonucleotides in smooth muscle cells is caused by a nonantisense mechanism.
- The structure and nucleotide sequence of the 5' end of the human c-myc oncogene.