AplicaÃÃo do anticorpo monoclonal 2F12 na pesquisa de anti- NTPase em lÃgrimas de indivÃduos portadores de toxoplasmose ocular em Recife- PE

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Toxoplasma gondii it is an important cause of posterior uveitis in immunocompetent individuals, leading to a partial or total vision loss, characterizing an important clinical form of the disease, the ocular toxoplasmosis. Currently, the laboratorial diagnosis of this illness is not satisfactory, leading to uncorrelation between the circulating antibodies levels and the ocular symptomatology, being common low antibodies titers in these patients. To assist the clinical diagnosis and differentiating toxoplasmic injury from those caused for another etiology, Asai et al (1983) produced an monoclonal antibody (Mab) 2F12, against a citosolic enzyme, the NTPase, present in tachyzoite form of parasite. This enzyme was detected in the blood of infected mice and allowed to identify the active phase of the parasite. This study aimed to investigate the prescence of NTPase in tears of individuals with ocular toxoplasmosis clinically active,using 2F12 monoclonal antibody as a tool for laboratorial diagnosis of posterior uveitis indiced by T.gondii. The NTPase determination was accomplishment by using dot blot test. Tears samples belonged to 21 patients with active posterior uveitis induced by T. gondii (APUPT) with gold standard lesions (the patients showed satellites lesions to the scarred lesions) and 20 healthy control subjects without lesions induced by T. gondii. The tears samples were incubated with disc of nitrocellulose for 18 hour at 4Â C, blocked, incubated with the Mab 2F12 during 1 hour, followed by a-mouse Igt biotin and avidin-peroxidase conjugates. The sensitivity of the test was 92,57%, specificity 55%, positive predictive value 63,31% and negative predictive value 90,48%. Fisherâs test demonstrated association between NTPase in APUT patients p=0,0005, considered significant p<0,05 and odds ratio was 21,444 (C.I. 95%:2, 726 a 219,21). Results show strong association between NTPase in tears, detected by 2F12 monoclonal antibody and active ocular toxoplasmosis

ASSUNTO(S)

anticorpo monoclonal ocular toxoplasmosis toxoplasmose ocular diagnosis diagnÃstico medicina monoclonal antibody ntpase ntpase

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