Aplicação de metodos fotoquimicos e bioquimicos na detecção do cancer

AUTOR(ES)
DATA DE PUBLICAÇÃO

1982

RESUMO

Studies on the excited species of biological systems suggested the possibility of using the "photobiochemistry in the dark" to analyse physiological situations in clinical research. The erythrocyte extracted enzyme, that metabolizes folic acid concomitantly with generation of singlet oxygen was analysed and its activity was calculated by measuring the emission of such species in several samples collected from normal individuals and cancerous patients. Concomitantly, the spontaneous emission of plasm and urine has been studied to verify the correlation between this phenomena and the enzyme activity in some pathological diseases. The nature of the excited species, present in urine and plasm have been investigated and it was shown that singlet oxygen and other activited species of oxygen (like OH•, O2•¨ etc) were important in the chemiluminescence of these fluids. The high activity of folic acid oxidase and the low chemiluminescence in the urine of cancerous patients suggest that in these pathological diseases a meaningful concentration of antioxidants would be eliminated through the urine. This inhibitory effect is similar to that observed in vivo with the ascorbid acid in the urine chemiluminescence from normal patients. The activity of folic acid oxidase: the inhibited chemiluminescence in the urine and the slight increase of chemiluminescence in the plasm of cancerous patients, led us to conclude that our methodology is importante in detecting pathological diseases as for example cancer.

ASSUNTO(S)

fotoquimica bioquimica clinica cancer

ACESSO AO ARTIGO

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