Application and Evaluation of the Interlaboratory Reproducibility of tRNA Intergenic Length Polymorphism Analysis (tDNA-PCR) for Identification of Streptococcus Species

AUTOR(ES)

Baele, Margo

FONTE

American Society for Microbiology

RESUMO

The discriminatory power, speed, and interlaboratory reproducibility of tRNA intergenic length polymorphism analysis (tDNA-PCR) combined with capillary electrophoresis was evaluated for the identification of streptococci. This method was carried out in three different laboratories under highly standardized conditions for 54 strains belonging to 18 different species. It was concluded that interlaboratory reproducibility of tDNA fingerprints produced by means of capillary electrophoresis was sufficiently high to permit the exchange between different laboratories and the construction of common libraries which can be consulted for comparison with fingerprints obtained independently in separate laboratories. In a second step, 17 other species were included in the study and examined in one of the participating laboratories. All Streptococcus species studied, except S. mitis, S. oralis, S. parasanguinis, S. pneumoniae, S. thermophilus, and S. vestibularis, showed distinguishable tDNA fingerprints. A database of well-characterized strains was constructed to enable computer-aided identification of unknown streptococcal isolates.

Documentos Relacionados

• Application of tRNA Intergenic Spacer PCR for Identification of Enterococcus Species
• Rapid and accurate identification of Staphylococcus species by tRNA intergenic spacer length polymorphism analysis.
• Rapid and Accurate Identification of Staphylococcus Species by tRNA Intergenic Spacer Length Polymorphism Analysis
• Application of PCR ribotyping and tDNA-PCR for Klebsiella pneumoniae identification
• Identification of Candida species by PCR and restriction fragment length polymorphism analysis of intergenic spacer regions of ribosomal DNA.