Application of the Intracellular Gamma Interferon Assay To Recalculate the Potency of CD8+ T-Cell Responses to Herpes Simplex Virus

AUTOR(ES)

Kumaraguru, Udayasankar

FONTE

American Society for Microbiology

RESUMO

Enumeration and characterization of herpes simplex virus (HSV)-specific CD8+ T lymphocytes are tedious and indirect. We quantitated antigen-specific CD8+ T cells during acute and secondary stages of HSV infection using intracellular gamma interferon production upon stimulation with virus or immunodominant peptide. Results show a substantial increase in the number of CD8+ T cells which was otherwise underestimated with the conventional limiting dilution analysis.

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