Application of the solid phase C1q and Raji cell radioimmune assays for the detection of circulating immune complexes in glomerulonephritis.
AUTOR(ES)
Tung, K S
RESUMO
The C1q solid phase and Raji cell radioimmune assays were used to determine the frequency of detectable circulating immune complexes in patients with glomerulonephritis. In this study, 46% of 56 patients with glomerulonephritis had evidence of circulating immune complexes. More important, circulating immune complexes were associated with some, but not other, types of glomerulonephritis. Thus, immune complexes were detected in lupus glomerulonephritis (9/9 patients), rapidly progressive glomerulonephritis (5/6 patients), and acute nephritis (5/6 patients), but not in IgA-IgG glomerulonephritis (0/7 patients), or membranous glomerulonephritis (0/8 patients). The Raji cell radioimmune assay and the C1q solid phase radioimmune assay showed concordance of 79% in the detection of circulating immune complexes. Serial determinations, in general, showed either persistence of a negative or positive result of conversion of positive to negative.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=371737Documentos Relacionados
- Circulating Immune Complexes in Lyme Arthritis: DETECTION BY THE 125I-C1q BINDING, C1q SOLID PHASE, AND RAJI CELL ASSAYS
- Inhibition of direct binding of enzyme-conjugated antihuman IgG t C1q using dextran sulphate in solid phase assays for immune complexes.
- The Raji cell radioimmune assay for detecting immune complexes in human sera.
- Circulating and intra-articular immune complexes in rheumatoid arthritis: a comparative study of the C1q binding and monoclonal rheumatoid factor assays.
- Circulating immune complexes in subacute infective endocarditis and post-streptococcal glomerulonephritis.