Assay of Vitamins and Amino Acids With Cultured Tissue Cells and Antimetabolites

AUTOR(ES)

Savchuck, W. B.

RESUMO

A survey of growth responses of tissue-culture cells to vitamins and amino acids was undertaken to explore the potentialities of tissue culture in the assay of growth factors. An antagonist of the nutrient was included in each test system to improve its sensitivity. Addition of an antimetabolite was advantageous in the thiamine and phenylalanine assays. Tissue-culture assays of tryptophan and of phenylalanine supplemented with β-2-thienylalanine compared favorably with microbial assays, and may serve as confirmatory or supplementary test systems. The sensitivity of cultured tissue cells to minute amounts of a variety of physiologically active substances suggests their employment in hormone and toxic compound assays.

Documentos Relacionados

• Abnormal concentrations of B vitamins and amino acids in plasma and B vitamins in bile of rabbits with aflatoxicosis.
• Abnormal concentrations of B vitamins and amino acids in plasma, bile, and liver of chicks with aflatoxicosis.
• Role of Amino Acids and Vitamins in Nutrition of Mesophilic Methanococcus spp
• Regulation of Sulfate Uptake by Amino Acids in Cultured Tobacco Cells
• Posttranscriptional Control of Nitrate Reductase of Cultured Tobacco Cells by Amino Acids