Assembly intermediates among adenovirus type 5 temperature-sensitive mutants.
AUTOR(ES)
Edvardsson, B
RESUMO
Temperature-sensitive mutants from three different complementation groups, ts5, ts19, and 6s58, have been shown to accumulate assembly intermediates at the restrictive temperature. The polypeptide composition of these intermediates is similar to that of the wild type, including the precursor polypeptides pVI, pVII, and pVIII. ts5 and ts19 also contained cleaved precursors, indicating assembly into defective virions. The increase of infectious virus after temperature shift-down of ts19 and ts58 was rapid when compared with that of ts24, which does not accumulate intermediates, suggesting that intermediates formed at nonpermissive temperature may be processed to mature virus. However, shift-down experiments reveal that only a fraction of the intermediates are utilized for virus assembly and that degradation of intermediates occurs at the restrictive temperature.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=353977Documentos Relacionados
- Flagellar assembly in Salmonella typhimurium: analysis with temperature-sensitive mutants.
- DNA-Minus Temperature-Sensitive Mutants of Adenovirus Type 5 Help Adenovirus-Associated Virus Replication
- Genetic analysis of adenovirus type 2. I. Isolation and genetic characterization of temperature-sensitive mutants.
- Selection and Preliminary Characterization of Temperature-Sensitive Mutants of Type 5 Adenovirus 1
- Properties of Bacillus megaterium temperature-sensitive germination mutants.
