Assembly of empty capsids by using baculovirus recombinants expressing human parvovirus B19 structural proteins.
AUTOR(ES)
Brown, C S
RESUMO
Empty parvovirus B19 capsids were isolated from insect cells infected with a recombinant baculovirus expressing parvovirus B19 VP2 alone and also with a double-recombinant baculovirus expressing both VP1 and VP2. That VP2 alone can assemble to form capsids is a phenomenon not previously observed in parvoviruses. The stoichiometry of the capsids containing both VP1 and VP2 was similar to that previously observed in parvovirus B19-infected cells. The capsids were similar to native capsids in size and appearance, and their antigenicity was demonstrated by immunoprecipitation and enzyme-linked immunosorbent assay with B19-specific antibodies.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=240632Documentos Relacionados
- Formation of empty B19 parvovirus capsids by the truncated minor capsid protein.
- A genetically engineered cell line that produces empty capsids of B19 (human) parvovirus.
- Cryo-electron microscopy studies of empty capsids of human parvovirus B19 complexed with its cellular receptor.
- Human Parvovirus B19
- Antibody response to human parvovirus B19 in patients with primary infection by immunoblot assay with recombinant proteins.